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df Did you know that the word “duchess” is an acronym of the words of Deucalion and Pyrrha, the couple from Greek Mythology who were hurled from the Ark? No? Well, nor did I until I started researching the begats of Luther. After a couple of years of research, I still couldn’t find any reason why the names of Luther’s parents would be so intertwined.
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In German the name “Saxe-Wittenberg” is spelled like this, “Sachs-Wittenburg” with a “Z.
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A new method for determination of xylitol in urine is described. Xylitol was derivatized with trifluoroacetic acid to the corresponding trifluoroacetate. The trifluoroacetate was used as the ionizing reagent in selected ion flow tube mass spectrometry (SIFT-MS). This allowed to detect xylitol in urine, without prior clean-up of the sample. To validate the new method, two sets of urine samples were analysed. First, 26 urine samples of pregnant women obtained from an Obstetric Department. Second, 28 urine samples of healthy volunteers. The sensitivity and the linearity of the method were validated to 18,500,000. As internal standard, 1,2,4-butanetriol-d(3) was used. The results obtained in the pregnant women and in the volunteers were in agreement with the values obtained by capillary gas chromatography. The new method offers a simple tool for fast and accurate determination of xylitol in urine.Separation of enzymes from lysed muscle fibers and changes in their kinetic properties.
Biochemical data have been collected on the activities of the mitochondrial and soluble enzymes of sarcolemma from reinnervated rat extensor digitorum longus muscle fibers after 2-4 days of in-vitro culture. At this time, after removal of donor axons and insertion of foreign axons in the muscle, the numbers of myofibrils and mitochondria per fiber increase. The increased number of mitochondria correlates with a decrease in specific activities of mitochondrial enzymes and with an increase in the number of “inactive” mitochondria. These observations suggest that there is a large pool of “silent” mitochondria with regard to those functions for which they are suited. These mitochondria could be a normal part of the biochemical machinery of a muscle fiber, and the imbalance in their number between active and inactive mitochondria may represent a normal example of cell communication. The kinetic properties of muscle enzymes of “labile” and “static” constituents of the regenerating fiber have been studied. The “labile” fractions
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