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To evaluate the expression levels and roles of cyclooxygenase (COX)-2 in human hepatocellular carcinoma (HCC), a subset of 30 HCCs was selected as a training set and the expression pattern of COX-2 in two other sets of HCC was subsequently evaluated. The COX-2 expression was assessed by immunohistochemical staining, and the relationship between COX-2 expression and the clinicopathologic parameters was evaluated. Meanwhile, mRNA expression levels of COX-2 in the frozen biopsy specimens and paraffin-embedded tissues were also determined by quantitative reverse-transcription polymerase chain reaction (RT-PCR). A total of 26 of 30 HCC specimens (86.6%) were immunopositive for COX-2, and 44.4% (6/13) of moderately differentiated HCCs showed strong expression of COX-2, which was significantly higher than that (7.1%, 2/28) in the well differentiated HCCs (P 5 cm, P =.04), a higher number of tumor cells (P =.02), and a higher number of tumor cells (P =.02) than the patients with a negative or weak COX-2 expression. In addition, the COX-2 immunopositive patients had a worse disease-free survival (
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